| 微流控芯片电泳分离血清高密度脂蛋白亚类的研究 |
| |
| 引用本文： | 郑慧斐,丛辉,王惠民,金庆辉,赵建龙. 微流控芯片电泳分离血清高密度脂蛋白亚类的研究[J]. 生物化学与生物物理进展, 2010, 37(2): 218-223 |
| |
| 作者姓名： | 郑慧斐 丛辉 王惠民 金庆辉 赵建龙 |
| |
| 作者单位： | 南通大学附属医院检验医学中心;中国科学院上海微系统与信息技术研究所; |
| |
| 基金项目： | 国家高技术研究发展计划(863)(2007AA042106); 国家重点基础研究发展计划(973)(2007CB714502,2007CB936000); 卫生部重大专项(2009ZX10004-301,2009ZX10004-505); 江苏省医学重点建设学科资助(XK200723); 南通市社会发展计划(S8919,S2009012)资助项目; 上海市科委项目(08110700200,0752nm021)~~ |
| |
| 摘 要： | 描述了一种微流控芯片电泳快速分离血清高密度脂蛋白(high density lipoprotein,HDL)亚类的方法.利用自制的微流控芯片,结合激光诱导荧光检测系统,40mmol/L Tricine、50mmol/L甲基葡胺(MEG)、0.2mmol/LSDS(pH8.5)为样品缓冲液,40mmol/L Tricine、50mmol/LMEG、0.01mmol/L SDS(pH8.5)为分离缓冲液,4min内HDL3和HDL2两种亚类得到基线分离.该法操作过程简单,重复性较佳,测试费用低廉,在临床HDL亚类的检测中具有较好的应用前景.
|
| 关 键 词： | 微流控芯片电泳 高密度脂蛋白 分析 |
| 收稿时间： | 2009-08-25 |
| 修稿时间： | 2009-12-04 |
Analysis of serum high density lipoprotein subclasses by electrophoresis on microfluidic chip |
| |
| ZHENG Hui-Fei,CONG Hui,WANG Hui-Min,JIN Qing-Hui and ZHAO Jian-Long. Analysis of serum high density lipoprotein subclasses by electrophoresis on microfluidic chip[J]. Progress In Biochemistry and Biophysics, 2010, 37(2): 218-223 |
| |
| Authors: | ZHENG Hui-Fei CONG Hui WANG Hui-Min JIN Qing-Hui ZHAO Jian-Long |
| |
| Affiliation: | ZHENG Hui-Fei1,2),CONG Hui1),WANG Hui-Min1),JIN Qing-Hui2),ZHAO Jian-Long2)(1) Laboratory Medicine Center,Affiliated Hospital of Nantong University,Nantong 226001,China,2) Shanghai Institute of Microsystem , Information Technology,The Chinese Academy of Sciences,Shanghai 200050,China) |
| |
| Abstract: | A method that could separate the subclasses of high density lipoprotein(HDL) rapidly by microfluicic chip electrophoresis was reported.Combined with laser induced fluorescence detection system,using the microfluicic chip designed independent,using 40 mmol/L Tricine,50 mmol/L MEG and 0.2 mmol/L SDS(pH 8.5) as sample buffer solutions,using 40 mmol/L Tricine,50 mmol/L MEG and 0.01 mmol/L SDS(pH 8.5) as separation buffer solutions,the two subclasses,HDL3 and HDL2 could be separated in 4 min.HDL subclasses could... |
| |
| Keywords: | microfluidic chip electrophoresis high density lipoprotein analysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
|
点击此处可从《生物化学与生物物理进展》下载全文 |
|
