河蚌C-反应蛋白的分离纯化及其性质研究

用合成的CDPC-Sepharose-6B亲和层析吸附剂一步提纯河蚌C-反应蛋白(CRP)。纯化的CRP在SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和等电聚焦电泳鉴定时均显示为一条区带。其等电点(pI)为5.2,分子量约为310,000道尔顿,由六个亚基组成,亚基分子量约为51,000道尔顿。在280nm处的消光系数E_(1cm)~(1mg)/ml=1.2。河蚌CRP与C_多糖(CPS)发生的沉淀反应是钙依赖性的。本文对河蚌CRP的氨基酸组成及其它性质也作了研究。

PURIFICATION AND SOME PROPERTIES OF ANODONTA C-REACTIVE PROTEIN

A simple one-step procedure for isolation and purification of anodonta C-reactive protein(CRP) by using CDPC-Sepharose 6B resin has been developed.The purified protein showed a single band on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focussing (IEF).It reacted and precipitated with the pneumococcal C-polysaccharide (CPS) in the presence of Ca2+.The molecular weight of anodonta CRP is approximately 310,000 daltons.It is composed of six subunits with a Mr=51,000 as shown by SDS-PAGE.The absorption coefficient at 280 nm has a value of E1cm1mg/ml=1.2,and the pI is 5.2.The analytical results of amino acid composition and some other properties are also reported in this paper.