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Organisation of Photosystem I and Photosystem II in red alga Cyanidium caldarium: Encounter of cyanobacterial and higher plant concepts
Authors:Zdenko Gardian  Ladislav Bumba  Adam Schrofel  Jana Nebesarova  Frantisek Vacha
Institution:a Biological Centre, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 ?eské Budějovice, Czech Republic
b Institute of Physical Biology, University of South Bohemia, Zámek 136, 373 33 Nové Hrady, Czech Republic
c Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Praha 4, Czech Republic
d Department of Biochemistry, Faculty of Science, Charles University, Albertov 6, 128 43 Praha 2, Czech Republic
e Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, 370 05 ?eské Budějovice, Czech Republic
Abstract:Structure and organisation of Photosystem I and Photosystem II isolated from red alga Cyanidium caldarium was determined by electron microscopy and single particle image analysis. The overall structure of Photosystem II was found to be similar to that known from cyanobacteria. The location of additional 20 kDa (PsbQ′) extrinsic protein that forms part of the oxygen evolving complex was suggested to be in the vicinity of cytochrome c-550 (PsbV) and the 12 kDa (PsbU) protein. Photosystem I was determined as a monomeric unit consisting of PsaA/B core complex with varying amounts of antenna subunits attached. The number of these subunits was seen to be dependent on the light conditions used during cell cultivation. The role of PsaH and PsaG proteins of Photosystem I in trimerisation and antennae complexes binding is discussed.
Keywords:Chl  chlorophyll  DEAE  diethyaminoethyl  DM  d-maltoside" target="_blank">n-dodecyl-β-d-maltoside  EM  electron microscopy  HL  high light  LHCI  light-harvesting complex of Photosystem I  LHCII  light-harvesting complex of Photosystem II  LL  low light  MES  2-morpholinoethanesulfonic acid  OEC  oxygen evolving complex  PSI  Photosystem I  PSII  Photosystem II  SDS-PAGE  polyacrylamide gel electrophoresis in a presence of sodium dodecylsulfate
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