Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa L.) |
| |
Authors: | Xifeng Chen Jianwei Pan Jing Cheng Guanghuai Jiang Yang Jin Zhimin Gu Qian Qian Wenxue Zhai Bojun Ma |
| |
Affiliation: | (1) College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China;(2) Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China;(3) China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China; |
| |
Abstract: | Spotted leaf 5 (spl5), a lesion mimic mutant, was first identified in rice (Oryza sativa L.) japonica cv. Norin8 in 1978. This mutant exhibits spontaneous disease-like lesions in the absence of any pathogens and resistance to rice blast and bacterial blight; however, the target gene has not yet been isolated. In the present study, we employed a map-based cloning strategy to finely map the spl5 gene. In an initial mapping with 100 F2 individuals (spl5/spl5) derived from a cross between the spl5 mutant and indica cv. 93-11, the spl5 gene was located in a 3.3-cM region on chromosome 7 using six simple sequence repeat (SSR) markers. In a high-resolution genetic mapping, two F2 populations with 3,149 individuals (spl5/spl5) were derived from two crosses between spl5 mutant and two indica cvs. 93-11 and Zhefu802 and six sequence-tagged site (STS) markers were newly developed. Finally, the spl5 gene was mapped to a region of 0.048 cM between two markers SSR7 and RM7121. One BAC/PAC contig map covering these markers’ loci and the spl5 gene was constructed through Pairwise BLAST analysis. Our bioinformatics analysis shows that the spl5 gene is located in the 80-kb region between two markers SSR7 and RM7121 with a high average ratio of physical to genetic distance (1.67 Mb/cM) and eighteen candidate genes. The analysis of these candidate genes indicates that the spl5 gene represents a novel class of regulators controlling cell death and resistance response in plants. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|