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Bongkrekic acid and atractyloside inhibits chloride channels from mitochondrial membranes of rat heart |
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| Authors: | Lubica Malekova Miroslav Ferko Olga Krizanova Adam Szewczyk |
| Institution: | a Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34 Bratislava, Slovak Republic b Institute for Heart Research, Slovak Academy of Sciences, 83334 Bratislava, Slovakia c Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland |
| Abstract: | The aim of this work was to characterize the effect of bongkrekic acid (BKA), atractyloside (ATR) and carboxyatractyloside (CAT) on single channel properties of chloride channels from mitochondria. Mitochondrial membranes isolated from a rat heart muscle were incorporated into a bilayer lipid membrane (BLM) and single chloride channel currents were measured in 250/50 mM KCl cis/trans solutions. BKA (1-100 μM), ATR and CAT (5-100 μM) inhibited the chloride channels in dose-dependent manner. The inhibitory effect of the BKA, ATR and CAT was pronounced from the trans side of a BLM and it increased with time and at negative voltages (trans-cis). These compounds did not influence the single channel amplitude, but decreased open dwell time of channels. The inhibitory effect of BKA, ATR and CAT on the mitochondrial chloride channel may help to explain some of their cellular and/or subcellular effects. |
| Keywords: | BLM bilayer lipid membrane Popen open probability of single channel Erev reversal potential SMP submitochondrial particles BKA bongkrekic acid ATR atractyloside CAT carboxyatractyloside CsA cyclosporine A MA myristic acid TBT tributyltin MPTP mitochondrial permeability transition pore IMAC inner membrane anion channel |
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