Expression of storage-protein genes during soybean seed development |
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Authors: | D W Meinke J Chen R N Beachy |
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Institution: | (1) Plant Biology Program, Department of Biology, Washington University, 63130 St. Louis, MO, USA |
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Abstract: | Mature seeds of Glycine max (L.) Merr. contain two major storage proteins, a glycosylated 7S protein (conglycinin) and a non-glycosylated 11S protein (glycinin). Accumulation of these proteins and their mRNAs during seed development in cv. Provar was studied by SDS polyacrylamide gel electrophoresis and by Northern (DNA-RNA) hybridization. The 11S acidic and basic subunits and the 7S ![agr](/content/ht1j561021107tx6/xxlarge945.gif) and subunits began to accumulate 18–20 d after pollination, shortly after the termination of cell division in developing cotyledons, whereas the 7S and 11S A-4 subunits were not detected until one to two weeks later, during the maturation phase of development. Messenger RNAs for 7S and 11S proteins were first detected 14–18 d after pollination, several days before the accumulation of storage proteins. Extracts from embryonic axes contained reduced levels of the 7S subunit, very little 11S protein, no detectable 7S or 11S A-4 subunits, and an additional 7S subunit not found in cotyledons. Soybean axes and cotyledons therefore differ in their synthesis of seed storage proteins.Abbreviations cDNA
complimentary DNA
- mRNA
messenger RNA
- SDS
sodium dodecyl sulfate |
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Keywords: | Embryonic axis Glycine mRNA accumulation Seed development Storage proteins |
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