Permeabilization of Pichia pastoris for glycolate oxidase activity |
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| Authors: | S. Gough M. Deshpande M. Scher J.P.N. Rosazza |
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| Affiliation: | (1) Center for Biocatalysis and Bioprocessing, University of Iowa, Iowa, 52242, USA |
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| Abstract: | Maximum activity of glycolate oxidase was obtained from a recombinant Pichia pastoris by permeabilization with 0.1% benzalkonium chloride for 60 min at room temperature. After treatment, intracellular glycolate oxidase activity increased 10-fold with respect to untreated cells. |
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| Keywords: | glycolate oxidase permeabilization Pichia pastoris yeast |
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