Suspension culture of hematopoietic stem cells in stirred bioreactors |
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| Authors: | Kwon Joon Kim Byung-Soo Kim Mi-Jung Park Hong-Woo |
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| Affiliation: | (1) Department of Chemical Engineering, Hanyang University, Seoul, Korea;(2) Department of Cell Biology, Asan Institute for Life Sciences, University of Ulsan, Asan Medical Center, Song Pa P.O. Box 145, Seoul, 138736, Korea |
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| Abstract: | Hematopoietic stem cells have applications in bone marrow transplantations for the treatment of hematopoietic disorders. When murine hematopoietic stem cells were cultured in 50 ml stirred bioreactors for 14 d, stem-cell-antigen-1 positive cells (hematopoietic primitive progenitor cells) and long-term culture-initiating cells (hematopoietic stem cells) grew by 5-fold and 4-fold, respectively. These results show the possibility of growing hematopoietic stem cells using a stirred bioreactor. |
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| Keywords: | hematopoietic stem cell stirred bioreactor suspension culture |
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