Three inhibitor types from wheat endosperm are differentially active against α-amylases of Lepidoptera pests |
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Authors: | C. Gutierrez,G. Garcia-Casado,R. S nchez-Monge,L. Gomez,P. Casta era,G. Salcedo |
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Affiliation: | (1) Unitad Estructural de Fitopatología, Centro de Investigaciones Biológicas-CSIC, Velazquez 144, 28006 Madrid, Spain;(2) Departmento de Bioquímica, E.T.S. Ingenieros Agrónomos, Ciudad Universitaria, 28040 Madrid, Spain |
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Abstract: | Crude α-amylase preparations from seven Lepidoptera pests were susceptible to inhibition by salt-soluble proteins of bread wheat (Triticum aestivum L.) endosperm. Protein fractions that corresponded to tetrameric, dimeric, and monomeric wheat α-amylase inhibitors, were decreasingly effective against the insect α-amylase activity. To further confirm these results, purified inhibitors were tested against an α-amylase preparation fromEphestia kuehniella (Zeller). This preparation showed decreased activity when increasing amounts of an heterotetrameric inhibitor (reconstituted from its isolated subunits WTAI-CM2, -CM3 and -CM16) were assayed. Activity was only partially inhibited by homodimeric (WDAI-1, synonym 0.53; WDAI-2, synonym 0.19) and monomeric (WMAI-1, synonym 0.28) inhibitors. |
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Keywords: | LepidopteraEphestia kuehniella α -amylase Triticum aestivum endosperm α -amylase inhibitors |
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