| 小麦白粉病生防菌G-1菌株原生质体诱变育种 |
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| 引用本文： | 肖敦科,陈家任,姬超宏,葛绍荣.小麦白粉病生防菌G-1菌株原生质体诱变育种[J].工业微生物,2010,40(5):54-57. |
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| 作者姓名： | 肖敦科 陈家任 姬超宏 葛绍荣 |
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| 作者单位： | 1. 四川大学生命科学学院生物资源与生态环境教育部重点试验室,成都,610064
2. 中国科学院成都生物研究所,成都,610041 |
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| 摘 要： | 以链霉菌G-1(Streptomyces sp.G-1)为出发菌株,通过研究菌株G-1原生质体形成与再生的条件,发现该菌株在含0.5%甘氨酸的菌丝体培养基中经过二次培养后,所得菌丝体用2 mg/mL溶菌酶在30℃下处理90 min,可获得大量原生质体,其再生率可达8.2%。菌株G-1的原生质体经紫外诱变和宁南霉素抗性筛选后,得到一高产突变株G-1-125,其有效组分A的产量达到794mg/L,较出发菌株提高了180%。
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| 关 键 词： | 小麦白粉病 链霉菌G-1 原生质体诱变 抗性筛选 |
Protoplast mutagenesis breeding of Streptomyces sp. G-1 against Blumeria graminis |
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| XIAO Dun-ke,CHEN Jia-ren,JI Chao-hong,GE Shao-rong.Protoplast mutagenesis breeding of Streptomyces sp. G-1 against Blumeria graminis[J].Industrial Microbiology,2010,40(5):54-57. |
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| Authors: | XIAO Dun-ke CHEN Jia-ren JI Chao-hong GE Shao-rong |
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| Institution: | ( Key Labratory of Bio-Resources and Eco-envirnnment of Ministry of Education , College of Life Science, Sichuan University, Chengdu 610064 ; 2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041 ) |
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| Abstract: | In order to screen a high-yielding strain of Streptomyces sp. G-1, factors affecting protoplasts preparation and regeneration were investigated. Results showed that the mycelia eutured in a hypha medium containing 0.5 % glyeine were processed with 2 mg/mL lysozyme at 30 ℃. for 90 minutes, the regeneration frequency of protoplasts could reach up to 8.2 %. Then the protoplasts were mutated by UV radiation and spread on regeneration plates containing 4 mg/mL ningnanmycin. After incubation and selection, a mutant G-1-125 was obtained . Its yield was as high as 794 mg/L, increased by 180% compared with that of the starting strain. |
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| Keywords: | wheat powdery mildew Streptomyces sp G-1 protoplast mutagenesis resistance screening |
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