Inhibitors of glycosphingolipid biosynthesis reduce transepithelial electrical resistance in MDCK I and FRT cells |
| |
Authors: | Leung Lawrence W Contreras Ruben G Flores-Maldonado Catalina Cereijido Marcelino Rodriguez-Boulan Enrique |
| |
Affiliation: | Margaret M. Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, New York 10021, USA. |
| |
Abstract: | Madin-Darby canine kidney (MDCK)I and Fisher rat thyroid (FRT) cells exhibit transepithelial electricalresistance (TER) values in excess of 5,000 · cm2. When these cells wereincubated in the presence of various inhibitors of sphingolipidbiosynthesis, a >5-fold reduction of TER was observed without changesin the gate function for uncharged solutes or the fence function forapically applied fluorescent lipids. The localization of ZO-1 andoccludin was not altered between control and inhibitor-treated cells,indicating that the tight junction was still intact. Furthermore, thecomplexity of tight junction strands, analyzed by freeze-fracturemicroscopy, was not reduced. Once the inhibitor was removed and thecells were allowed to synthesize sphingolipids, a gradual recovery ofthe TER was observed. Interestingly, these inhibitors did not attenuatethe TER of MDCK II cells, a cell line that typically exhibits valuesbelow 800 · cm2. These resultssuggest that glycosphingolipids play a role in regulating theelectrical properties of epithelial cells. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
| 点击此处可从《American journal of physiology. Cell physiology》浏览原始摘要信息 |
|
点击此处可从《American journal of physiology. Cell physiology》下载全文 |
|