Cytochemical localization of ouabain-sensitive,K+-dependent p-nitrophenylphosphatase and Ca++-stimulated adenosine triphosphatase activities in human parotid and submandibular glands |
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Authors: | M Cossu M S Lantini P Puxeddu A Riva |
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Institution: | (1) Istituto di Anatomia Umana Normale, Università di Cagliari, Via Porcell 2, I-09100 Cagliari, Italy;(2) Clinica Otorinolaringoiatrica, Università di Cagliari, Cagliari, Italy |
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Abstract: | Summary K+-dependent p-nitrophenylphosphatase (pNPPase) and Ca++-stimulated adenosine triphosphatase (ATPase) activities were studied in human parotid and submandibular glands using cytochemical methods at the ultrastructural level. In both glands, only the striated-duct epithelium showed K+-pNPPase reaction product, thereby indicating the localization of Na+, K+-ATPase. The precipitate was concentrated on the deep invaginations of the basolateral plasma membranes, in close association with their cytoplasmic surface. Ca++-ATPase activity was also found on the basolateral plasma membranes, but two striking differences from the K+-pNPPase distribution were observed: firstly, Ca++-ATPase appeared in both acinar and ductal cells, and secondly, it was localized on the outer side of the plasma membranes. |
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