Detection of antibiotic resistance genes associated with methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci in hospital air filter dust by PCR |
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| Authors: | Christopher N. Drudge Sigmund Krajden Richard C. Summerbell James A. Scott |
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| Affiliation: | (1) Dalla Lana School of Public Health, University of Toronto, 223 College Street, Toronto, ON, M5T 1R4, Canada;(2) St. Joseph’s Health Centre, 30 The Queensway, Toronto, ON, M6R 1B5, Canada;(3) Sporometrics, 219 Dufferin St, Suite 20C, Toronto, ON, M6K 1Y9, Canada;(4) Present address: School of Geography & Earth Sciences, McMaster University, Hamilton, ON, Canada; |
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| Abstract: | Dust from the pre-filters of stand-alone hospital isolation room air cleaners was tested by PCR for the presence of antibiotic resistance genes associated with methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci. Resistance genes for three classes of antibiotics (aac(6′)-aph(2″), ermA, and mecA) were detected in multiple samples, indicating the presence of genetic material and likely cells of multidrug-resistant MRSA and other staphylococci in hospital air and that stand-alone air cleaners can reduce airborne levels of these contaminants. Screening for vanA was negative. Our results further suggest that dust may serve as an important reservoir of genetic elements that can confer drug resistance. |
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