Development and characterization of 21 polymorphic microsatellite loci in the aphidophagous gall midge, Aphidoletes aphidimyza (Diptera: Cecidomyiidae) |
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| Authors: | Norihide Hinomoto Tomomi Higaki Junichiro Abe Masafumi Yamane Eizi Yano |
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| Affiliation: | (1) Plant Protection Division, NARO Agricultural Research Center (NARC), National Agriculture and Food Research Organization (NARO), Tsukuba Ibaraki, 305-8666, Japan;(2) Natural Enemies Laboratory, Insect Interaction Research Unit, Division of Insect Sciences, National Institute of Agrobiological Sciences, Tsukuba Ibaraki, 305-8634, Japan;(3) Lowland Crops Research Division, NARO Western Region Agricultural Research Center (WARC), National Agriculture and Food Research Organization (NARO), Fukuyama Hiroshima, 721-8514, Japan;(4) Entomological Laboratory, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan |
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| Abstract: | We have developed and characterized 21 microsatellite markers in the aphidophagous gall midge Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae). All 21 loci tested were polymorphic: the number of alleles ranged from 2 to 17. Allelic richness and observed heterozygosities were higher in females than in males. Several loci had no heterozygosity in males, suggesting that the loci were located on sex chromosomes or E-chromosomes, common to cecidomyiids. The high polymorphism detected in this study suggests the markers will be of value in analyzing genetic structure of field populations. |
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