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ESTimating plant phylogeny: lessons from partitioning
Authors:Jose EB de la Torre  Mary G Egan  Manpreet S Katari  Eric D Brenner  Dennis W Stevenson  Gloria M Coruzzi  Rob DeSalle
Institution:(1) Department of Biology, New York University, 100 Washington Sq East, New York, NY 10003, USA;(2) American Museum of Natural History, Central Park West @79th St., New York, NY 10024, USA;(3) New York Botanical Garden Bronx, 200th Street and Kazimiroff Boulevard, Bronx, NY, 10458, USA
Abstract:

Background  

While Expressed Sequence Tags (ESTs) have proven a viable and efficient way to sample genomes, particularly those for which whole-genome sequencing is impractical, phylogenetic analysis using ESTs remains difficult. Sequencing errors and orthology determination are the major problems when using ESTs as a source of characters for systematics. Here we develop methods to incorporate EST sequence information in a simultaneous analysis framework to address controversial phylogenetic questions regarding the relationships among the major groups of seed plants. We use an automated, phylogenetically derived approach to orthology determination called OrthologID generate a phylogeny based on 43 process partitions, many of which are derived from ESTs, and examine several measures of support to assess the utility of EST data for phylogenies.
Keywords:
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