Influence of growth phase and carbon source on the content of rubredoxin in Acinetobacter calcoaceticus |
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Authors: | Reiner Claus Otmar Asperger Hans-Peter Kleber |
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Affiliation: | (1) Bereich Biochemie der Sektion Biowissenschaften der Karl-Marx-Universität Leipzig, Talstr. 33, DDR-701 Leipzig, Deutsche Demokratische Republik |
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Abstract: | The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin from Acinetobacter calcoaceticus. The formation of rubredoxin increased with time up to the end of the logarithmic phase when n-alkanes were the sole carbon source. After growth of Acinetobacter calcoaceticus on non-hydrocarbon substrates, rubredoxin was not detected. |
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Keywords: | n-Alkane degradation Acinetobacter calcoaceticus Rubredoxin Radioimmunoassay |
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