Effects of nitrogen source on betacyanin accumulation and growth in suspension cultures of Phytolacca americana |
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Authors: | Masaaki Sakuta Tsutomu Takagi Atsushi Komamine |
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Institution: | Biological Inst., Faculty of Science, Tohoku Univ., Sendai 980, Japan;Advanced Research Lab., Hitachi, Ltd., Kokubunji, Tokyo 185, Japan;Nishiki Research Lab., Kureha Chemical Industry Co., Ltd, Iwaki, Fukushima 974, Japan |
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Abstract: | In suspension cultures of Phytolacca americana L., betacyanin accumulation per cell increased with increasing total nitrogen concentration (initial NH+4:NO?3 ratio 1:2) in the range 0–40 m M and then remained almost constant in the range 40–80 m M . Increasing ammonium increased growth while betacyanin accumulation was reduced. On the other hand, betacyanin accumulation increased when nitrate was increased while growth was almost constant in the concentration range examined. A time-course study of ammonium and nitrate concentration changes in the medium showed that betacyanin accumulation was associated with nitrate uptake. |
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Keywords: | Ammonium betacyanin cell culture nitrate nitrogen metabolism Phytolacca americana |
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