| 一种女贞叶抗冻蛋白的分离纯化 |
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| 引用本文： | 刘尚 廖祥儒 张建国 苗会娟. 一种女贞叶抗冻蛋白的分离纯化[J]. 植物学报, 2007, 24(4): 505-510 |
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| 作者姓名： | 刘尚 廖祥儒 张建国 苗会娟 |
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| 作者单位： | 江南大学生物工程学院教育部工业生物技术重点实验室, 无锡 214122 |
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| 摘 要： | 根据抗冻蛋白与冰结合的特性, 利用碎冰从女贞(Ligustrum lucidum)叶提取液中分离出抗冻蛋白。结果表明, 通过碎冰吸附、凝胶过滤和离子交换层析可以获得4个组分的蛋白质, 其中的1个经鉴定具有热滞活性。在蛋白质浓度为5 mg.mL-1时, 它的热滞活性(thermal hysteresis activity, THA)值为0.678°C, 对其进行全波长扫描(200-1 000 nm)发现在975nm处有吸收峰; 该蛋白亲水性氨基酸含量较高。
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| 关 键 词： | 抗冻蛋白 性质 冰结合 女贞 纯化 |
| 收稿时间： | 2007-01-05 |
| 修稿时间： | 2007-01-18 |
Purification of an Antifreeze Protein from Ligustrum lucidum Leaves and Its Partial Characteristics |
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| Shang Liu Xiangru Liao Jianguo Zhang Huijuan Miao. Purification of an Antifreeze Protein from Ligustrum lucidum Leaves and Its Partial Characteristics[J]. Bulletin of Botany, 2007, 24(4): 505-510 |
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| Authors: | Shang Liu Xiangru Liao Jianguo Zhang Huijuan Miao |
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| Affiliation: | Educational Ministry Key Laboratary of Industrial Biotechnology, School of Biotechnology, Southern Yangtze University, Wuxi 214122, China |
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| Abstract: | According to the ice-binding characteristic, antifreeze proteins in the leaves of Ligustrum lucidum were isolated by adsorption to ice pieces at 0°C. Using Sephadex G-100 gel filtration and DEAE cellulose-52 anion exchange chromatography, we obtained 4 protein fractions, including a 36 kDa protein, identified as antifreeze proteins by differential scanning calorimetry. The THA value of the protein was approximately 0.678 °C when the protein concentration was 5 mg.mL-1. The maximal absorption peak was 975 nm after full-spectrum scanning from 200 to 1 000 nm. The content of hydrophilic amino acids was relatively higher than that of the other proteins. |
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| Keywords: | ; |
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