Engineering aspects of immobilized lipases on esterification: A special emphasis of crowding,confinement and diffusion effects |
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| Authors: | Surendra Kumar Parashar Sunil Kumar Srivastava N. N. Dutta Vijay Kumar Garlapati |
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| Affiliation: | 1. Department of Chemical Engineering/Chemistry, Jaypee University of Engineering and Technology, Guna, Madhya Pradesh, India;2. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India |
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| Abstract: | Cross‐linked enzyme crystal (CLEC) and sol‐gel entrapped pseudomonas sp. lipase were investigated for the esterification of lauric acid with ethanol by considering the effects of reaction conditions on reaction rate. The activation energy for the reaction was estimated to be 1097.58 J/mol and 181.75 J/mol for sol‐gel and CLEC entrapped lipase respectively. CLEC lipase exhibited a marginal internal diffusion effect on reaction rate over sol‐gel lipases and found to be interesting. The overall reaction mechanism was found to conform to the Ping Pong Bi Bi mechanism. The higher efficiency of sol‐gel lipases over CLEC lipases in esterification reaction is mainly due to the combined effects of crowding, confinement and diffusional limitations. |
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| Keywords: | CLEC lipase Confinement Crowding Diffusion Ethyl laurate Sol‐gel lipase |
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