A Point Mutation in the Coat Protein Gene Affects Long-Distance Transport of the Tobacco Mosaic Virus |
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Authors: | Koshkina T E Baranova E N Usacheva E A Zavriev S K |
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Institution: | (1) Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Moscow, 127550, Russia |
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Abstract: | A mutation resulting in substitution of positively charged Lys53 with negatively charged Glu in the coat protein was introduced in the infectious cDNA copy of the genome of wild-type tobacco mosaic virus strain U1. Kinetic analysis of long-distance virus transport in plants showed that systemic spread of the mutant virus was delayed by 5–6 days as compared with the wild-type one. On evidence of RNA sequencing in the mutant progeny, Glu50 of the coat protein was substituted with Lys after passage I to compensate for the loss of the positive charge at position 53. Electron microscopy revealed atypical inclusions (rodlike structures, multiple electron-dense globular particles) in the nuclear interchromatin space of leaf mesophyll cells infected with the mutant but not with the wild-type virus. |
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Keywords: | tobacco mosaic virus coat protein kinetics of long-distance virus transport |
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