In vivo immunogold labeling confirms large-scale chromatin folding motifs |
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Authors: | Kireev Igor Lakonishok Margot Liu Wenqiu Joshi Vishwas N Powell Rick Belmont Andrew S |
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Institution: | Department of Cell and Developmental Biology, University of Illinois, Urbana-Champaign, 601 S. Goodwin Ave., Urbana, Illinois 61801, USA. |
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Abstract: | The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP-proliferating cell nuclear antigen (PCNA). |
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