Genome-wide mapping of allele-specific protein-DNA interactions in human cells |
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Authors: | Maynard Nathaniel D Chen Jing Stuart Rhona K Fan Jian-Bing Ren Bing |
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Institution: | Ludwig Institute for Cancer Research, University of California, San Diego, 9500 Gilman Ave., La Jolla, California 92037, USA. |
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Abstract: | We describe a high-throughput method, named ChIP-SNP, for the identification of allele-specific protein-DNA interactions throughout the human genome. ChIP-SNP combines chromatin immunoprecipitation (ChIP) with whole-genome single nucleotide polymorphism (SNP) genotyping microarray analysis. We demonstrated that it can be used to accurately identify allele-specific binding of RNA polymerase II (RNAP) in the human fibroblast genome, uncovering imprinted genes and other allele-specific binding events. ChIP-SNP will facilitate the study of mechanisms of allele-specific gene expression. |
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