Hydrophilic C terminus of Salicornia europaea vacuolar Na+/H+ antiporter is necessary for its function |
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Authors: | GUANGXIA WU GANG WANG JING JI XIAOWEI TIAN HAILING GAO QING ZHAO JING LI YURONG WANG |
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Affiliation: | 1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China 2. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, People’s Republic of China 3. Yaohua High School, Tianjin, 300040, People’s Republic of China
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Abstract: | Plant vacuolar Na+/H+ antiporters play important roles in cellular ion homeostasis, vacuolar pH regulation and sequestration of Na+ ions into the vacuole. Previous research showed that hydrophilic C-terminal region of Arabidopsis AtNHX1 negatively regulates the Na+/H+ transporting activity. In this study, we truncated the hydrophilic C terminus of a vacuolar Na+/H+ antiporter gene from Salicornia europaea (SeNHX1) to generate its derivative, SeNHX1- ΔC. Expression of SeNHX1 and SeNHX1- ΔC in yeast mutant showed that SeNHX1 significantly improved the tolerance to NaCl; however, the expression of SeNHX1- ΔC enormously decreased the tolerance to NaCl. Overall, these results suggest that the hydrophilic C-terminal region of SeNHX1 is required for Na+/H+ exchanging activity of SeNHX1. |
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