The effect of heating on the respiration of MC7 and D23 tumour tissue |
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| Institution: | 1. Service d''Hématologie Clinique, Institut de Cancéro-Hématologie, CHU de Brest, Brest, France;2. Service d''Hématologie Biologique Hôpital Tenon, Hôpitaux Universitaires de l''Est Parisien, APHP, Paris, France;3. Research Group “Cancer, Hémostase et Angiogenèse” INSERM UMR_S 938, Centre de Recherche Saint-Antoine, Faculté de Médecine, Institut Universitaire de Cancérologie, Sorbonne Universités, Paris, France;4. Service de Médecine Interne, Maladies Infectieuses et du Sang, CH de Cornouaille, Quimper, France;5. Laboratoire d''Hématologie, CHU de Bordeaux, Bordeaux, France;6. INSERM, Univ Brest, CHU de Brest, UMR 1304, GETBO, Brest, France;7. Département de Médecine Interne et de Pneumologie, CHU de Brest, Brest, France;8. Laboratoire d''Hématologie, CHU de Brest, Brest, France;9. FIM, France Intergroupe des néoplasies Myéloprolifératives, France;1. Department of Medical Oncology, the Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China;2. Department of Guangdong Key Laboratory of Liver Disease Research, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;3. Department of Interventional Radiology, the Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China;4. Department of Project, Forevergen Biosciences Co., Guangzhou 510300, China;2. Center for Biomarker Research in Medicine, Graz, Austria;3. Department of Medical Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA;4. Department of Pharmacy, Pharmaceutical Biology, Saarland University, Saarbrücken, Germany;5. Liver Tumor Translational Research Program, University of Texas Southwestern Medical Center, Dallas, USA;11. Institute of Medical Engineering, Graz University of Technology, Graz, Austria;12. Institute of Molecular Biosciences, University of Graz, Graz, Austria;8. AG VABOS, Department of Cranio-Maxillofacial Surgery, Univerity of Muenster, Germany;9. Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria;112. Department IFA-Tulln, University for Natural Resources and Applied Life Sciences Vienna, Vienna, Austria;123. Institute of Laboratory Animal Science, University of Veterinary Medicine Vienna, Vienna, Austria;84. Institute of Biology, University of Leipzig, Leipzig, Germany;95. Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany;1111. Medical University Vienna, Vienna, Austria;1212. Department of Pathology, Medical Faculty, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany |
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| Abstract: | The sensitivity of the tissue respiration of two tumours and liver slices to heat has been studied. The tumour tissue is sensitive to in vitro preincubation at temperatures above 43°C, whereas liver slices were less temperature sensitive. The inclusion of 1 mM tetracaine during preincubation sensitizes the tumour tissue to heating. In vivo heating of the tumour tissue at 44°C for 1h was not inhibitory of respiration when subsequently measured at 37°C. Mitochondria isolated from the D23 hepatoma tissue showed coupled respiration, however mitochondria isolated from in vivo heated tumour did not show coupled respiration. It contrast to mitochondria isolated from unheated tissue, these mitochondria lacked cristae and contained electron-dense granules, indicators of damage. The lack of effect of an in vivo heat-dose, known to cause tumour regression, on respiration and the reports that ATP levels are unaffected by such heating, suggests that cell respiration is not a primary lesion in cellular heat injury. This implies that the observed impairment of mitochondrial function following in vivo heating is best explained if the heating sensitized the mitochondria to subsequent damage during isolation. |
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