A comparative study of clothing factors from the venom of Agkistrodon acutus collected in China and Taiwan |
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| Institution: | 1. National Laboratory for Development of Endangered Drug Resources in Northwest China, College of Life Sciences, Shaanxi Normal University, Xi''an, Shaanxi, 710062, China;2. Innovation Center for Qinba Regions Sustainable Development, College of Life Sciences, Shaanxi Normal University, Xi''an, Shaanxi, 710062, China;3. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China;4. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan;5. Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan |
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| Abstract: | - 1.1. Two clotting factors, Cf-1(C) and Cf-2(C) were isolated from Agkistrodon acutus (collected in China) venom by gel filtration, ion-exchange chromatography and affinity chromatography. Using the same procedure, two clotting factors, Cf-1(T) and Cf-2(T), were isolated from Agkistrodon acutus (collected in Taiwan) venom and their characteristics were compared with Cf-1(C) and Cf-2(C).
- 2.2. Molecular weights of Cf-1(C), Cf-2(C), Cf-1(T) and Cf-2(T) were determined to be 44,000, 70,000, 25,000 and 44,000 respectively. The factors were not immunologically related.
- 3.3. The four clotting factors possessed tosyl-l-arginine methyl ester (TAME) hydrolyzing activity and coagulated fibrinogen to fibrin. Only Aα chain was cleaved when fibrinogen was incubated with each factor.
- 4.4. Agkistrodon acutus is not classified by geographical location, however it is obvious that venom components vary between the Chinese and Taiwanese forms.
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