Digestive proteases in the midgut gland of the atlantic blue crab,Callinectes sapidus |
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| Affiliation: | 1. Ministry of Agriculture Shanghai Scientific Observing and Experimental Station of Argo-Environment and Arable Land Conservation, Eco-environmental Protection Institute of Shanghai Academy of Agricultural Sciences, Shanghai 201403, PR China;2. Coastal Ecosystems Research Station of the Yangtze River Estuary, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200438, PR China;3. Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA;1. Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy;2. Allgemeine & Spezielle Zoologie, Institut für Biowissenschaften, Universität Rostock, Universitätsplatz 2, 18055 Rostock, Germany;3. The Swire Institute of Marine Science and The School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong;4. Department of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino 50019, Italy |
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| Abstract: | - 1.1. Digestive proteases from the midgut gland of male Atlantic blue crabs, Callinectes sapidus, were investigated. Tentative identities of proteolytic enzymes were determined with synthetic substrates and inhibitors.
- 2.2. Trypsin, chymotrypsin, carboxypeptidase A and B and leucine aminopeptidase activities were found and quantified.
- 3.3. Activity against Succinyl-(Ala)3-nitroanalide was also found. This as yet unidentified enzyme has a mol. wt of about 26,000 and has elastolytic activity.
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