Ionic dependence and vanadate sensitivity of (Na+, K+)-ATPase isolated from branchial sac of ascidians |
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| Institution: | 1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China;2. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;3. Zanzibar Fisheries and Marine Resources Research Institute, Zanzibar 2789, Tanzania;1. Department of Animal, Aquaculture and Range Sciences, Sokoine University of Agriculture, P.O. Box 3004, Morogoro, Tanzania;2. Section for Zoophysiology, Department of Biology, Aarhus University, Aarhus C, Denmark;3. National Institute of Aquatic Resources, Section for Aquaculture, Technical University of Denmark, North Sea Science Park, Hirtshals, Denmark |
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| Abstract: | - 1.1. Ion dependence and vanadium-induced inhibition on branchial sac ATPase in five species of ascidian Phlebobranchiata (vanadium-accumulating) and Stolidobranchiata (iron-accumulating) were studied.
- 2.2. The ATPase was obtained from the microsomal fraction, which was prepared from each ascidian branchial sac.
- 3.3. The ATPase was dependent on Mg2+ and activated by exogenous Na+ + K+.
- 4.4. Ouabain inhibited the ATPase activity in vitro, 10 μM to 100 μM vanadate, in vitro, suppressed the (Na+, K+)-ATPase.
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