ELISA measurement of immunochemical cross-reactions among Lathyrus lectins as an assessment of their phylogenetical relationship |
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| Affiliation: | 1. Department of Anatomy and Cell Biology, Paracelsus Medical University, Nuremberg and Salzburg, Prof.-Ernst-Nathan Str. 1, 90419 Nuremberg, Germany;2. Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058 Erlangen, Germany;3. Department of Biosciences, Paris Lodron University Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria;4. Department of Otorhinolaryngology, Head and Neck Surgery, Charité- Universitätsmedizin, Luisenstr. 13, 10117 Berlin, Germany;1. Department of Chemistry, University of Calcutta, 92, A. P. C. Road, Kolkata 700 009, India;2. Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India |
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| Abstract: | The immunochemical cross-reactions among Lathyrus and other Vicieae lectins have been quantified by using an ELISA (enzyme-linked immunosorbent assay) technique, in order to assess their phylogenetical relationship. The data are consistent with those arising from the comparison of both the amino acid compositions of the lectins and the amino acid sequences of their light and heavy subunits. They confirm that two-chain lectins from the tribe of Vicieae are phylogenetically closely related. |
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