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Comparative studies on the contents of vitamin D3, 25-hydroxy vitamin D3 and 7-dehydrocholesterol in fish liver
Affiliation:1. Institute of Food Research and Product Development (IFRPD), Kasetsart University, 50 Ngamwongwan Rd., Chatuchak, Bangkok 10903, Thailand;2. Western Region Agricultural Research Center, National Agriculture and Food Research Organization (WARC/NARO), 1-3-1 Senyu, Zentsuji, Kagawa 765-8508, Japan;3. Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan;4. Tohoku Agricultural Research Center, National Agriculture and Food Research Organization (TARC/NARO), 297 Uenodai, Kariwano, Daisen, Akita 019-2112, Japan;5. Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 2-1-1 Kannondai, Tsukuba, Ibaraki 305-8602, Japan;1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;2. Pôle Actifs Végétaux, Institut de Recherche Pierre Fabre, 3 avenue Hubert Curien, 31035 Toulouse Cedex 1, France;3. USR CNRS-Pierre Fabre No. 3388 ETaC, Centre de Recherche et Développement Pierre Fabre, 3 avenue Hubert Curien, 31035 Toulouse Cedex 01, France;1. Instituto de Investigación Sanitaria Biodonostia, Área de Neurociencias, San Sebastian, Spain;2. CIBERNED, Madrid, Spain;3. Departamento de Neurociencias, Universidad del País Vasco (UPV/EHU), Leioa-Erandio, Spain;4. Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain;5. Institut des Maladies Neurodégénératives, CNRS UMR 5293, Université de Bordeaux, Bordeaux, France;6. Laboratoire de Neurosciences Expérimentales et Cliniques, INSERM U1084, Université de Poitiers, Poitiers, France;7. Ikerbasque Foundation, Bilbao, Spain;8. Basque Center on Cognition, Brain and Language, San Sebastian, Spain;9. Clínica Universidad de Navarra, Centre for Applied Medical Research (CIMA), Pamplona, Spain
Abstract:
  • 1.1. The contents of vitamin D3, 25-hydroxyvitamin D3 (25-OH-D3) and 7-dehydrocholesterol (7-DHC) in 22 kinds of fish liver samples were determined by a high-performance liquid chromatographic (HPLC) method.
  • 2.2. Vitamin D3 was detected in all fish liver samples, but its contents varied from 84 to 264,000 ng/g wet tissue. The liver of fish belonging to Carangidae and Scombridae contained large amounts of the vitamin and therefore we deduced that vitamin D3 levels in liver might have some relations with taxonomical positions of fishes.
  • 3.3. 25-OH-D3 was detected in 7 out of 22 kinds of fish liver samples, while 7-DHC was in 14 out of 22. The contents of the two sterols were generally much lower than those of vitamin D3 and there was no special relationship between the contents of the sterols and the vitamin.
Keywords:
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