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| 卡介苗荚膜阿拉伯甘露聚糖的提纯鉴定及其免疫调节功能的初步研究 | |
| 引用本文： | 邓国英,刘欣,杨淑凤.卡介苗荚膜阿拉伯甘露聚糖的提纯鉴定及其免疫调节功能的初步研究[J].中国微生态学杂志,2022,34(5):540-543. |
| 作者姓名： | 邓国英 刘欣 杨淑凤 |
| 作者单位： | 大连医科大学基础医学院微生物学教研室，辽宁 大连 116044 |
| 基金项目： | 国家自然科学基金(81801981)； |
| 摘 要： | 确定卡介苗(BCG)中荚膜中糖成分阿拉伯甘露聚糖(AM)的功能。 本研究从我国目前普遍使用的BCG中, 利用氯仿甲醇抽提方法分离纯化荚膜AM, 经AM单克隆抗体鉴定后, 腹腔注射BCG致敏小鼠, 体内外评价AM的免疫调节功能。 AM能在小鼠体内增强BCG诱导的迟发型超敏反应( BCG的荚膜AM是潜在的保护性抗原, 并为优化BCG提供理论依据。 |
| 关 键 词： | 荚膜多糖 阿拉伯甘露聚糖 卡介苗 |
| 收稿时间： | 2021-04-22 |
| 修稿时间： | 2021-05-18 |
Purification and identification of arabinomannan from BCG and its immunoregulation in BCG-sensitized mice |
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| DENG Guo-ying,LIU Xin,YANG Shu-feng.Purification and identification of arabinomannan from BCG and its immunoregulation in BCG-sensitized mice[J].Chinese Journal of Microecology,2022,34(5):540-543. | |
| Authors: | DENG Guo-ying LIU Xin YANG Shu-feng |
| Institution: | Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, China |
| Abstract: | ObjectiveTo explore the function of capsular arabinomannan(AM) located in the outmost layer of Bacille Calmette-Guérin(BCG).MethodsThe capsular AM of BCG was isolated using chloroform-methanol extraction, and identified by using AM specific monoclonal antibody, then boosted twice by peritoneal injection in BCG-sensitized mice.ResultsThe identified AM was used to boost BCG primed-mice. The treated mice showed a greater delayed-type hypersensitivity response in vivo(P < 0.01), as well as stronger Th1 responses, including higher level of IFN-γ and TNF-α(P < 0.05).ConclusionCapsular AM in BCG is a potential protective antigen. It provided key insight for future mycobacterial vaccine optimization. |
| Keywords: | Capsular polysaccharide Arabinomannan Bacille Calmette-Guérin |
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