Ribosomal RNA gene variation in diploid and tetraploid Tolmiea menziesii |
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| Institution: | 1. Department of Botany, Washington State University, Pullman, WA 99164, U.S.A.;2. Bailey Hortorium, Cornell University, Ithaca, NY 14853, U.S.A.;1. Princeton Neuroscience Institute, Princeton University, Princeton, NJ, United States;2. Department of Brain and Cognitive Sciences and Center for Visual Science, University of Rochester, Rochester, NY, United States;1. Giraffe Conservation Foundation, PO Box 86099, Windhoek, Namibia;2. Senckenberg Biodiversity and Climate Research Centre, Senckenberganlage 25, 60325 Frankfurt am Main, Germany;3. Wildlife Conservation Society, Bronx Zoo, 2300 Southern Blvd., Bronx, NY 10460, USA;4. Museum of Zoology, Senckenberg Dresden, Königsbrücker Landstrasse 159, 01109 Dresden, Germany;5. Institute for Ecology, Evolution & Diversity, Goethe University, Max-von-Laue-Strasse 13, 60439 Frankfurt am Main, Germany;1. Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA;2. Genetics Institute, University of Florida, Gainesville, FL 32610, USA;3. Department of Biology, University of Florida, Gainesville, FL 32611, USA;4. Department of Plant Biotechnology and Bioinformatics, Ghent University and Department of Plant Systems Biology, VIB, B-9052, Ghent B-9052, Belgium;5. Bioinformatics Institute Ghent, Ghent University, B-9052, Ghent B-9052, Belgium;6. Genomics Research Institute, University of Pretoria, Pretoria 0028, South Africa;1. Centre for Functional Biodiversity, School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg 3209, South Africa;2. Ezemvelo KZN Wildlife, P O Box 13053, Cascades, Pietermaritzburg, 3202, South Africa;1. UMR 8079 Ecologie Systématique Evolution, Univ. Paris Saclay, CNRS, AgroParisTech, 362 rue du Doyen André Guinier, 91400, Orsay, France;2. UMR 7324 CNRS CITERES, 33 allée Ferdinand de Lesseps, 37200, Tours, INSA Centre Val de Loire, 8 rue de la chocolaterie, 41000, Blois, France |
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| Abstract: | Evidence from gross morphology, karyology and flavonoid chemistry suggests that Tolmiea menziesii is one of the clearest examples of autopolyploidy in natural populations. To provide additional data regarding the origin of the tetraploid cytotype of Tolmiea, both the 5S and 18S-25S ribosomal RNA genes were studied at the restriction enzyme level. Using restriction enzymes that cut once per repeat, the lengths of the 5S and 18S-25S ribosomal genes were estimated in diploids and tetraploid plants. There appear to be no consistent differences between diploids and tetraploids for the repeat length of the 18S-25S ribosomal genes. Furthermore, there is no significant repeat length heterogeneity within tetraploid plants for these genes. In addition, no differences in repeat length of the 5S genes were observed among the diploid and tetraploid plants analysed. The homogeneity observed among diploid and tetraploid plants for repeat length of the 5S and 18S-25S ribosomal genes is consistent with the hypothesis that the tetraploid cytotype is of autopolyploid origin. |
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