Immunoaffinity purification of a diuretic hormone from the nervous ventral cord of the migratory locust |
| |
| Institution: | 1. Laboratoire de Neuroendocrinologie, UA CNRS 1138, Université de Bordeaux I, France;2. Immunotech, Marseille, France;1. The University of Sydney, Faculty of Science, School of Psychology, Sydney, New South Wales, Australia;2. The University of Sydney, Brain and Mind Centre, Sydney, New South Wales, Australia;1. Department of Systems Physiology, University of the Ryukyus Graduate School of Medicine, Okinawa, Japan;2. Department of Systems Physiology, University of the Ryukyus, Faculty of Medicine, Okinawa, Japan |
| |
| Abstract: | - 1.1. An antibody raised against the mammal arginine-vasopressin (AVP) is used to immunopurify a neurohormone, structurally related to AVP, which enhances the excertion of the primary urine from the locust Malpighian tubules.
- 2.2. The best procedure of immunopurification is developed by testing with different solvents.
- 3.3. This procedure is applied to purify a semi-crude extract from 1000 suboesophageal and thoracic ganglia, the main source of hormone.
- 4.4. The interest of this technique in the purification of biological materials, when only minute amounts are available, is discussed.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
