Resonance Raman spectroscopy of polynucleotides |
| |
Authors: | B Jollès L Chinsky A Laigle |
| |
Institution: | Laboratoire Curie, Institut Curie, 11, rue Pierre et Marie Curie, 75231 Paris Cedex 05, France |
| |
Abstract: | Resonance Raman Spectroscopy allows a selective study of the bases of DNA and therefore of the interactions of these bases with ligands. This technique is also sensitive to structural modifications. We show here that, first, the structures of native poly(dA-dT).poly(dA-dT) and poly(dA).poly(dT) are not the same and that, secondly, it is possible to characterize the B----Z transition of poly(dG-dC).poly(dG-dC). The study of the Raman hypochromism during the thermal denaturation of the polynucleotides reveals that the stacking of the adenines in poly(dA).poly(dT) is near that observed in poly(rA) but differs of this stacking in poly(dA-dT).poly(dA-dT). The enhancement of the intensity of the guanine line at 1193 cm-1 and of the cytosine lines at 780 cm-1, 1 242 cm-1 and 1268 cm-1 as well as the shift of the guanine line at low frequency should allow to characterize a small proportion of base pairs in Z form in any DNA. |
| |
Keywords: | ultraviolet resonance Raman spectroscopy polynucleotides hypochromism Z Form spectroscopie Raman de résonance dans l'UV polynucléotides hypochromisme forme Z |
本文献已被 ScienceDirect 等数据库收录! |
|