|
|||||
|
|
Evaluation of radiation-induced chromosomal aberrations in human peripheral blood lymphocytes in vitro results of an IAEA-coordinated programme |
|
| Authors: | M Bianchi NO Bianchi JG Brewen KE Buckton L Fabry P Fischer PC Gooch M Kucerova A Lonard RN Mukherjee U Mukherjee S Nakai AT Natarajan G Obe F Palitti J Pohl-Rüling HG Schwarzacher D Scott T Sharma E Takahashi C Tanzarella PPW van Buul |
| Institution: | 1 IMBICE, La Plata, Argentina 2 Molecular and Applied Genetics Laboratory, Allied Corporation, Morristown, NJ 07960, U.S.A. 3 MRC, Clinical and Population Cytogenetics Units, Western General Hospital, Edinburgh, Great Britain 4 Mammalian Genetics Laboratory, Department of Radiobiology, CEN-SCK, B-2400, Mol, Belgium 5 Institute of Cancer Research, University of Vienna, Vienna, Austria 6 Biology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, U.S.A. 7 Genetics Department of Post-Graduate Medical School, Prague, Czechoslovakia 8 International Atomic Energy Agency, Vienna, Austria 9 Histology and Embryology Institute, University of Vienna, Vienna, Austria 10 National Institute of Radiological Sciences, 9-1, 4-Chome, Anagawa, Chiba-Shi, Japan 11 Department of Radiation Genetics and chemical Mutagenesis, State University of Leiden, Leiden, The Netherlands a J.A. Cohen Interuniversity Institute of Radiopathology and Radiation Protection, Leiden, The Netherlands 12 Institute of Genetics, Free University of Berlin, D-1000, Berlin 33, F.R.G. 13 Institute of Genetics, University of Rome, Rome, Italy 14 Division of Biophysics, University of Salzburg, Salzburg, Austria 15 Christ Hospital and Holt Radium Institute, Paterson Laboratories, Manchester, Great Britain 16 Department of Zoology, Banares Hindu University, Varanasi 221005, India |
| Abstract: | The results of an IAEA coordinated programme on radiation induced chromosomal aberrations in human peripheral blood lymphocytes in vitro are presented. In a master experiment, a whole blood sample from one donor was irradiated with 200 R of X-rays. Different fixation times from 46 to 82 h were used. The progression of cells into mitosis was monitored by BrdUrd incorporation. 14 investigators took part in the scoring of chromosomal aberrations. The main conclusions of this study are: (1) The mean frequencies of aberrations changed with fixation time. (2) The number of cells scored as aberrant by different laboratories was very similar, but there was variability in the number of aberrations scored per aberrant cell. (3) The differences in the frequencies of aberrations between laboratories were minimal when the scoring was restricted to the first major peak of mitotic activity and sufficient cells were scored. It is concluded that using controlled experimental conditions, human peripheral blood lymphocytes can effectively be used as a reliable biological dosimeter for absorbed radiation dose. |
| Keywords: | Address all correspondence to A T Natarajan Sylvius Laboratories Wassenaarseweg 72 AL 2333 LeidenM The Netherlands |
| 本文献已被 ScienceDirect 等数据库收录！ | |