Adrenoferredoxin-binding peptide of NADPH-adrenoferredoxin reductase |
| |
| Authors: | I Hamamoto K Kurokohchi S Tanaka Y Ichikawa |
| |
| Institution: | Department of Biochemistry, Kagawa Medical School, Japan. |
| |
| Abstract: | The amino-acid sequence at the adrenoferredoxin-binding site of NADPH-adrenoferredoxin reductase ferredoxin:NADP+ oxidoreductase, EC 1.18.1.2) from bovine adrenocortical mitochondria was investigated chemically. NADPH-adrenoferredoxin reductase has an essential lysine residue at the adrenoferredoxin-binding site. A polypeptide at the adrenoferredoxin-binding site was isolated by high-pressure liquid chromatography from NADPH-adrenoferredoxin reductase modified with pyridoxal 5'-phosphate and cleaved with cyanogen bromide. The amino-acid sequence of the adrenoferredoxin-binding peptide was identified. The peptide accounted for 95% of the sugar content of the NADPH-adrenoferredoxin reductase. |
| |
| Keywords: | |
|
|
