Callus and suspension culture ofSilybum marianum. Biosynthesis of proteins with clotting activity |
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| Authors: | P Fevereiro J M S Cabral M M R Fonseca J M Novais M Salomé S Pais |
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| Institution: | (1) Departamento de Biologia Vegetal, Faculdade de Ciências de Lisboa, 1294 Lisboa Codex, Portugal;(2) Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, 1000 Lisboa, Portugal |
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| Abstract: | Summary
Silybum marianum plants contain a proteinase with clotting activity which is used for the production of soft cheese. The influence of the concentrations of phytohormones (2,4-D and kinetin), of aminoacids and sucrose on the growth rate and intracellular protein content of calli and suspension cultures was studied. The clotting: proteolytic activity ratio was compared with that of a microbial rennet. |
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