The incorporation of precursors into Drosophila larval cuticle |
| |
| Institution: | 1. Developmental Biology Center, University of California, Irvine, CA 92717, U.S.A.;2. Department of Developmental and Cell Biology, University of California, Irvine, CA 92717, U.S.A.;1. Food Quality and Design Group, Wageningen University and Research, 6700AA, Wageningen, the Netherlands;2. Department of Food Science and Technology, Chinhoyi University of Technology, P Bag, 7724, Chinhoyi, Zimbabwe;1. KU Leuven, Department of Microbial and Molecular Systems (M2S), Lab4Food, Technology Campus Geel, Kleinhoefstraat 4, B-2440 Geel, Belgium;2. KU Leuven, Leuven Food Science and Nutrition Research Centre (LFoRCe), Leuven, Belgium;3. Department of Food Technology and Nutrition, CAES, Makerere University, P.O. Box, 7062 Kampala, Uganda;1. The Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao, 266003, PR China;2. Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China;1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA;1. Department of Animal Science, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa;2. Fisheries Management Branch, Department of Agriculture, Forestry and Fisheries, Roggebaai, 8012, South Africa;3. Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, 39 Kessels Rd, Coopers Plains 4108, Australia |
| |
| Abstract: | Incorporation of tritiated leucine, tyrosine and glucosamine into the integument of larval Drosophila melanogaster was followed by electron-microscope autoradiography. Tritiated leucine, tyrosine, and glucosamine were incorporated into the endocuticle by apposition, giving rise to a distinct band of label in the endocuticle at a level which depended on the time between labelling and fixation. The labelled amino acids, but not glucosamine, were also detected in the epicuticle and both above and below the distinct labelled band in the endocuticle. The results indicate that the epicuticle grows within the third instar by intussusception of new materials which are transported from the epidermal cells through the endocuticle to the epicuticle. Breakdown of cuticle which was radioactively labelled by feeding larvae tritiated precursors was also followed by autoradiography. The results indicate that the breakdown products from the old cuticle may be reutilized in the synthesis of new cuticle. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
