Virulence of mutants and revertants of Metarhizium anisopliae var. anisopliae toward Rhodnius prolixus |
| |
| Institution: | 1. DBM/CCEN, Federal University of Paraiba, 58000 Joāo Pessoa, PB, Brazil;2. Department of Genetics and Evolution, Institute of Biosciences, Estadual University of Campinas, 13100 Campinas, SP, Brazil;1. Department of Entomology, Federal University of Viçosa, Brazil;2. Department of Agronomy, Federal Rural University of Pernambuco, Brazil;3. Department of Statistics, Physics and Mathematics, Federal University of São João Del-Rei, Brazil;4. Institute of Biological Sciencies, Federal University of Minas Gerais, Brazil;1. Graduate School of Science and Engineering, University of Toyama, Toyama, Japan;2. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan;3. Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan;1. EMBRAPA Genetic Resources and Biotechnology, Parque Estação Biológica, W5 Norte, 70770-917 Brasilia, DF, Brazil;2. EMBRAPA Rice and Beans, Rodovia GO-462, km 12, Zona Rural, 75375-000, Santo Antonio de Goias, GO, Brazil;1. Key Laboratory of Concrete and Prestressed Concrete Structures of the Ministry of Education, Southeast University, Nanjing 210096, China;2. Department of Mechanical and Biofunctional Systems, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan;1. Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Rockefellerova 10, 10 000 Zagreb, Croatia;2. Quality Control Department, Institute of Immunology, Svetonedeljska Cesta 14, 10 431 Sveta Nedelja, Croatia;3. Department of Laboratory Medicine, Karolinska University Hospital Huddinge, 14 186 Stockholm, Sweden;4. Department of Histology and Embryology, School of Medicine, Braće Branchetta 20, 51000 Rijeka, Croatia |
| |
| Abstract: | The derivation of mutants of Metarhizium anisopliae var. anisopliae which had lost or showed reduced ability to produce extracellular amylases, lipases, and proteases was studied by the enzymatic index. Reversion induced by ultraviolet light was also studied. The virulence of mutants and revertant strains was evaluated in bioassays against third-instar nymphs of Rhodnius prolixus. The mutant strains for amylase and lipase showed enzymatic indices equal to one, but this was not the case for the proteolytic mutants. The revertant strains were phenotypically similar to the parental strains. The virulence of amylolytic and lipolytic mutants was reduced in relation to the parental strain, whereas the proteolytic mutants did not show any significant reduction. Virulence was restored in all revertant strains. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
