A Naturally Occurring Variant of Porcine Mx1 Associated with Increased Susceptibility to Influenza Virus In Vitro |
| |
| Authors: | Emiko Nakajima Takeya Morozumi Kenji Tsukamoto Tomomasa Watanabe Graham Plastow Tadayoshi Mitsuhashi |
| |
| Affiliation: | (1) Animal Gene Function Laboratory, Department of Physiology and Genetic Regulation, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan;(2) Second Research Division, STAFF Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan;(3) Department of Virology, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-0856, Japan;(4) Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan;(5) Sygen International, 2 Kingston Business Park, Kingston Bagpuize, Oxon, OX13 5FE, UK |
| |
| Abstract: | Mx1 has been implicated in resistance to the influenza virus. We have now identified four alleles of the Mxl gene in domesticated breeds of pigs. Two of the alleles encode deletion variants (a 3-bp deletion in exon 13 and an 11-bp deletion in exon 14), which might be expected to interfere with Mx activity. The porcine Mxl genes corresponding to wild type, the 3-bp deletion mutant, and the 11-bp deletion mutant were cloned and expressed in NIH3T3 cells, and the antiviral activity for influenza virus was assayed. Virus yield was observed to be 10–100-fold greater with the 11-bp deletion allele than that for wild type and the 3-bp deletion alleles. The results suggest that the 11-bp deletion type is lacking antiviral activity able to contribute to the interference of influenza virus replication. |
| |
| Keywords: | porcine Mx1 influenza virus deletion allele |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
