High-performance affinity capture-removal of bacterial pyrogen from solutions |
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| Authors: | Jeak Ling Ding Yong Zhu Bow Ho |
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| Institution: | 1. Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China;2. Key Laboratory of Preclinical Study for New Drugs (Gansu Province), College of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China;1. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France;2. Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d’Investigació Germans Trias i Pujol, Universitat Autonoma de Barcelona, Badalona, Spain;3. Laboratório de Biologia Molecular Aplicada à Micobactérias, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil;4. Centre Muraz, Bobo-Dioulasso, Burkina Faso;5. Univ. Polytech, Bobo-Dioulasso, Burkina Faso;6. Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, Av. Brasil, 4365 - 20245, Rio de Janeiro, Brazil;7. Microbiology Department, Quaid-i-Azam University, Islamabad, Pakistan;8. Infectious Diseases and Tuberculosis Hospital, Affiliate of Vilnius University Hospital Santariskiu klinikos, Vilnius, Lithuania;9. National Center for Biotechnology, Astana, Kazakhstan;10. Universitat Autònoma de Barcelona. CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Spain |
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| Abstract: | Synthetic peptide S3Δ has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5–9 and 0–0.4 M NaCl, the affinity constant, KD ranged from 2·10−6 to 2·10−9 M−1. A novel affinity matrix based on peptide S3Δ was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of ≈100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. |
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| Keywords: | Pyrogen Lipopolysaccharides Endotoxins |
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