In vitro modulation of differentiation by calcium in organ cultures of human and murine epithelial tissue |
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Authors: | P G Sacks S M Parnes J C Price H Risemberg J C Goldstein M Marko D F Parsons |
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Institution: | (1) Center for Laboratories and Research, New York State Department of Health, Empire State Plaza, 12201 Albany, New York;(2) Division of Otolaryngology, Albany Medical Center, 12208 Albany, New York;(3) Newborn Services, Albany Medical Center, 12208 Albany, New York;(4) Present address: Dept. Tumor Biol., M.D.Anderson Hospital and Tumor Institute, 77030 Houston, Texas |
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Abstract: | Summary The differentiation of epithelial tissue in organ cultures of murine buccal mucosa, various human oral mucosa, and human newborn
foreskin was found to be dependent on the calcium concentration of the culture media. In low calcium medium (≤0.07 mM) epithelial differentiation was inhibited. The original stratifying layers separate and can be removed, producing a destratified
explant. Histologically such an explant consits of a dorsal epithelial layer of basal keratinocytes resting on an intact basal
lamina with subjacent stroma. At 0.01 mM calcium, the epithelial layer was one to two cells thick whereas at 0.07 mM it could be three or more layers in thickness with the most superficial cells being spread over the underlying cells. In
addition to differentiation, keratinocyte migration over the sides of the explant (epiboly) and epithelial proliferation as
determined by 3H]thymidine autoradiography were reduced by culture in low calcium medium. Redifferentiation occurs upon return to normal
calcium levels (1.8 mM); addition of hydrocortisone to low calcium media was found to facilitate this redifferentiation.
This investigation was supported by NIH Grant CA29255 from the National Cancer Institute, PHS/DHHS, and by NIH Grant RR01219
supporting the New York State High-Voltage Electron Microscope as a National Biotechnology Resource, awarded by the Division
of Research Resources, PHS/DHHS. |
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Keywords: | epithelium mucosa calcium differentiation proliferation organ cultures |
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