| 米根霉ME—F12发酵产富马酸的菌体形态控制 |
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| 引用本文： | 徐晴,高振,付永前,李霜,黄和. 米根霉ME—F12发酵产富马酸的菌体形态控制[J]. 生物加工过程, 2009, 7(2): 48-52 |
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| 作者姓名： | 徐晴 高振 付永前 李霜 黄和 |
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| 作者单位： | 1. 南京工业大学,制药与生命科学学院,南京,210009
2. 南京工业大学,制药与生命科学学院,南京,210009;江苏省工业生物技术创新中心,南京,211816 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，国家重点基础研究发展规划(973计划) |
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| 摘 要： | 丝状真菌发酵体系中菌体形态对产量有着重要影响。考察富马酸产生菌Rhizopus oryzae ME—F12种子培养过程中不同pH条件、孢子悬浮液密度以及CaCl2添加量对其形态的影响。结果表明，当控制种子培养液pH2．3～2．7、接种孢子的终密度为1．5×10^8～3．0×10^8／L和添加0．5g／LCaCl2时，培养可获得直径约为0．65mm光滑规整茵球，后继的产酸发酵中富马酸量高达58．9g／L。正交实验表明，pH是影响菌球形成的最主要因素，孢子液密度主要影响菌体生物量，而CaCl2则是菌球表面光滑度的主要影响因素。
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| 关 键 词： | 富马酸 形态 产量 米根霉 |
Morphology control of Rhizopus oryzae ME-F12 in fumaric acid production |
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| XU Qing,GAO Zhen,FU Yong-qian,LI Shuang,HUANG He. Morphology control of Rhizopus oryzae ME-F12 in fumaric acid production[J]. Chinese Journal of Bioprocess Engineering, 2009, 7(2): 48-52 |
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| Authors: | XU Qing GAO Zhen FU Yong-qian LI Shuang HUANG He |
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| Affiliation: | 1.College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009; 2. Jiangsu Provincial Innovation Center for Industrial Biotechnology, Nanjing 211816, China) |
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| Abstract: | The effect of pH, inoculum, and CaCl2 in the seed culture medium fumaric acid production by Rhizopus oryzae ME-F12 was investigated. When pH spore concentration in the flask was 1.5 × 10^8 to 3. 0 × 10^8 spores/L. By adding on the strain morphology and was between 2. 3 and 2. 7, the of CaCl2（0. 5 g/L） , the strain grew into the uniform and smooth pellet, and the diameter was less than 0. 65 mm. In this case, the concentration of fumaric acid was further increased to 58. 9 g/L. The orthogonal experiment including factors of pH, inoculum, and CaCl2 was carried out. The result showed that pH was the most significant factor for the morphology, the inoculum affected the mass of seed and CaCl2 affected the surface of pellets. |
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| Keywords: | fumaric acid morphology production Rhizopus oryzae |
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