Isolation and characterization of a cDNA clone for a novel serine-rich neutrophil protein. |
| |
| Authors: | P Bellavite F Bazzoni M A Cassatella K J Hunter J V Bannister |
| |
| Institution: | Institute of General Pathology, University of Verona, Italy. |
| |
| Abstract: | A cDNA expression library from pig blood neutrophils was immunoscreened with a rabbit antiserum raised against a 32 kDa neutrophil membrane phosphoprotein. Previous work indicated this protein as a component of the superoxide-forming NADPH oxidase enzyme complex (1,2). Only one cDNA clone (B+) was highly positive. The B+ clone contained a 1109 bp insert, with an open reading frame encoding for 284 amino acids. The deduced B+ amino acid sequence contained a 72 amino acid domain with proline and glutamine repeats and two domains extremely enriched with serine residues. The isolated cDNA hybridizes with a 3.1 kb mRNA expressed in pig and human leukocytes. |
| |
| Keywords: | |
|
|
