Reversible DIDS binding to Band 3 protein in human erythrocyte membranes |
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Authors: | Teresa Janas Tadeusz Janas |
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Institution: | 1. Department of Physics, Technical University, Podgórna 50, 65-246 Zielona Góra, Poland;2. Department of Biophysics, Pedagogical University, Aleja Wojska Polskiego 69, 65-762 Zielona Góra, Poland |
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Abstract: | Reversible binding of DIDS 4,4'-diisothiocyanato-2,2'-stilbenedisulphonate] to Band 3 protein, the anion exchanger located in erythrocyte plasma membrane, was studied in human erythrocytes. For this purpose, the tritiated form of DIDS (3H]DIDS) has been synthesized and the filtering technique has been used to follow the kinetics of DIDS binding to the sites on Band 3 protein. The obtained results showed monophasic kinetics both for dissociation and association of the 'DIDS-Band 3' complex at 0° C in the presence of 165 mM KCl outside the cell (pH 7.3). A pseudo-first order association rate constant k+1 |
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Keywords: | Band 3 Erythrocyte Membrane Stilbenedisulphonates Dissociation Rate Constant Association Rate Constant |
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