The structure of the efflux pump AcrB in complex with bile acid |
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| Authors: | David Drew Mirjam M. Klepsch Simon Newstead Ralf Flaig Jan-Willem De Gier |
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| Affiliation: | 1. Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London;2. Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden;3. Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Chilton, Oxfordshire, UK |
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| Abstract: | Gastrointestinal bacteria, like Escherichia coli, must remove bile acid to survive in the gut. Bile acid removal in E. coli is thought to be mediated primarily by the multidrug efflux pump, AcrB. Here, we present the structure of E. coli AcrB in complex with deoxycholate at 3.85 Å resolution. All evidence suggests that bile acid is transported out of the cell via the periplasmic vestibule of the AcrAB-TolC complex. |
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| Keywords: | Multidrug efflux protein AcrB bile acid E. coli structure |
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