On the mechanism of bilitranslocase transport inactivation by phenylmethylsulphonyl fluoride |
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Authors: | Sabina Passamonti Lucia Battiston Gian Luigi Sottocasa |
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Affiliation: | Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Universita degli Studi di Trieste, 1 34100 Trieste, Italy |
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Abstract: | Bilitranslocase is a plasma membrane carrier involved in the uptake of bilirubin and other organic anions from the blood into the liver cell. In the membrane, the carrier occurs as two interchangeable metastable forms, with high and low affinity for the substrates, respectively. The latter form can be specifically produced by either cysteine- or arginine modification. In liver plasma membrane vesicles, the serine-specific reagent phenylmethylsulphonyl fluoride is a partial inhibitor of bilitranslocase-mediated BSP transport rate. In this work, phenylmethylsulphonyl fluoride is shown to reduce the carrier maximal transport rate, without affecting its affinity for that substrate. In addition, it is found that the chemical modification caused by this reagent neither influences the equilibrium between the high- and the low-affinity forms nor prevents their free interconversion. From the effects of combined derivatizations of cysteine(s), arginine(s) and serine(s), it is concluded that the functionally relevant aminoacid residues lie in a close spatial arrangement. Also, in this study, the PMSF-modified serine(s) is shown to be involved in bilirubin binding by bilitranslocase. |
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Keywords: | Bilitranslocase Bilirubin Phenylmethylsulphonyl Fluoride Organic Anion Transport Liver Sinusoidal Plasma Membrane |
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