Deep-tank culture of blue-green algae in H2O and D2O

A 160-liter stainless steel algal growth tank has been constructed and has been used essentially continuously for over three years. Filamentous and unicellular blue-green algae as well as a photosynthetic bacterium have been cultured using both ordinary and heavy water (99.8 atom % ²H). By using a recycling technique, yields as high as 25 g/liter of ²H₂O have been obtained.