Model generation of viral channel forming 2B protein bundles from polio and coxsackie viruses |
| |
Authors: | George Patargias Thomas Barke Anthony Watts |
| |
Affiliation: | Biomembrane Structure Unit, Department of Biochemistry, Oxford University |
| |
Abstract: | 2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations a putative tetrameric bundle model of 2B is generated. The bundles show a pore lining motif of three lysines followed by a serine. The bundle is discussed in terms of different possible orientations of the helices in the membrane and the consequences this has on the in vivo activity of 2B. |
| |
Keywords: | 2B polio virus coxsackie virus membrane proteins assembly channels |
|