Plasma membrane redox system during HL-60 induced differentiation |
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Authors: | G. López-Lluch M. I. Burón F. J. Alcaín J. C. Rodríguez-Aguilera P. Navas |
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Affiliation: | (1) Departamento de Biologia Celular, Facultad de Ciencias, Universidad de Córdoba, Avda. San Alberto Magno s/n, E-14004 Córdoba, Spain |
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Abstract: | Summary HL-60 cells were induced to differentiate by exposure to TPA or 1,25-dihydroxyvitamin D3 (calcitriol). Induction with TPA was in parallel with a modulation of transmembrane redox system. After addition of 2 ng/m1 TPA, transient increases in ferricyanide reductase activity, NAD(H) intracellular levels and short-term response of NAD(H) to 0.4 mM ferricyanide were observed. The role of ascorbate on the differentiation induced by calcitriol also was studied. When HL-60 cells were exposed to 10–8 M calcitriol in the presence of 0.2 mM ascorbate, specific differentiation markers as NBT reduction or surface antigen CD11b increased significantly with respect to values obtained from treatments with calcitriol alone. |
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Keywords: | Redox system Differentiation HL-60 cell Calcitriol |
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