Overexpression of δ-Opioid Receptors in Recombinant Baculovirus-Infected Trichoplusia ni"High 5" Insect Cells |
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Authors: | Andrea Wehmeyer Rüdiger Schulz |
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Institution: | Institute of Pharmacology, Toxicology, and Pharmacy, University of Munich, Munich, Germany |
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Abstract: | Abstract: "High 5" cells derived from Trichoplusia ni ovaries were infected with baculovirus bearing the cDNA of the mouse δ-opioid receptor. The maximal binding capacity for the narcotic antagonist 3H]naltrindole was 1.4 pmol/mg of membrane protein, and that for the agonist 3H] d -penicillamine2, d -penicillamine5]enkephalin (DPDPE) was 0.3 pmol/mg. DPDPE proved highly potent in competing with its tritiated analogue at δ-receptors of NG108-15 hybrid cells and of High 5 and Sf9 insect cells. However, in insect cells the opioid was more than 100-fold less effective in competing with 3H]naltrindole as compared with the mammalian cells. This decline in potency was counteracted in a dose-dependent manner by exposure of High 5 membranes to the exogenous G protein Go, which increased the binding capacity for DPDPE. Functional studies revealed a dose-dependent inhibition (up to 30%) by opioids on forskolin-stimulated cyclic AMP synthesis, and this effect was potentiated by Go. Quantification of Gαo and Gαi disclosed striking differences between Sf9 and High 5 insect cells, both of which overexpressed the cloned δ-opioid receptor. Although no inhibitory G proteins were detected in membranes of Sf9 cells, High 5 cells contained 0.5 pmol of Gαo/mg of membrane protein, and a 20-fold higher concentration for Gαi. The distinct G-protein expression in insect cells may be considered an advantage for studying functions of G protein-coupled receptors. |
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Keywords: | Baculovirus "High 5" insect cells Sf9 δ-Opioid receptor Overexpression G proteins |
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